Analyzing differences in bacterial optical density. Clean the lab table before and after lab with the 10% bleach. Typically, this mayinclude a small throughthewall ethylene oxide gas cham ber for thecold sterilization ofheatsensitive ma terials 8 and a uv apparatus for. Industrial uv systems are used to treat large volumes of water that have become contaminated in. Uv lamps have many applications in the laboratory depending on the model used, including water testing, quality control inspection. Sop for monitoring uv light efficiency in laf and pass box. Uses for aa batteries not included for microbiology applications. Ultraviolet equipment is part of a germicidal light that can be used for quality inspection, uv curing, forensics detection, leak detection, bacterial identification, polymer curing, and more. This is the main page for the microbial control laboratory topic of an 8week introductory college microbiology class. Lepp second edition biol 142 general microbiology spring 2010. General microbiology laboratory manual biol 142 by paul w. Lab report 4 fr 1 control by heat and uv lab report. Laboratory manual and workbook in microbiology applications to patient care 7th edition josephine a. Laboratory ultraviolet light sources princeton university.
Laboratory exercises in microbiology cuny academic works. Ultraviolet radiation is often used to decontaminate the interior surfaces of. Basic uvvis theory, concepts and applications page 1 of 28 introduction ultraviolet and visible spectrometers have been in general use for the last 35 years and over this period have become the. Validation of the effectiveness of uv light in water. Microbiology laboratory manual prepared by abdelraouf elmanama. Laboratory ultraviolet light sources office of environmental health. The laboratory experience teaches the science behind the. Switch on the uv light for a period of 30 minutes so as to kill the germs, if any.
The purpose of this pharmaceutical microbiology manual pmm is to collectively. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Objectives to learn the effects of uv irradiation on different bacterial. Uv lamps have many applications in the laboratory depending on the model used, including water testing, quality control inspection, nondestructive testing, sanitation, uv curing, gel viewing, mineralogy, arson investigation, tlc, e. The dr6000 is a benchtop uvvis 190 1100 nm, split beam spectrophotometer that delivers top performance for both routine laboratory tasks and demanding applications. Uv experiment microbiology lab manual, study notes for. List of equipment apparatus used in microbiology laboratory. Uv lamp for microbiology vwr chemicals and laboratory. Module context this module introduces microbiological laboratory techniques to. Laboratory medicine, volume 38, issue 3, march 2007, pages. Ultraviolet radiation damage and repair live organisms.
Lethal effects plate out bacteria sterile swab cover entire plate take off lid place note card over half of plate label side exposed to uv place plate under hood for different amounts of time. Monitoring ultraviolet lamps in biological safety cabinets with cultures of. Basic practical microbiology why microbiology matters. Brighten up any room with this custom table lamp from lampinabox. This training presentation provides information about. Required lab supplies lab coat there is a limited supply of lab coats in the lab. Molbmicr 2021 fall semester 2010 university of wyoming. Mercury uv bulbs primarily emit light at 254 nanomers, while pulsed xenon uv bulbs emit uv light at several different germicidal wavelengths. When collecting uv lightexposed samples t1, t2, and. This manual has been written for a righthanded person. Molbmicr 2021 fall semester 2010 university of wyoming laramie, wyoming.
All of the devices produce uv light using either mercury uv bulbs that run continously or xenon uv bulbs that pulse. Fluorescent microbiology collection by microbiology. Biol 230 laboratory manual the grapes of staph table. Do not work in the bsc while the ultraviolet light is on.
Written by a microbiologist with over two decades of collective experience both teaching and coordinating lab courses, microbiology. While working, the uv light must be turned off and day light bulb is turned on. The eye is the most vulnerable organ to uv exposure. Pdf on oct 1, 2016, abu nayeem and others published book on microbiology. This uv lamp is designed for use with fluorocult culture media. The uv reflected from the light colored pages onto the students skin. Excitation by uvvis radiation results in electron transitions from bonding mos to their relative antibonding mos, and from nonbonding mos to either antibonding mos figure 4. View lab report lab report 4 fr from biol 2310 at georgia state university. Put on the uv protective glasses and then turn on the ultraviolet light source and. This ultraviolet lamp by uvp has a powerful 6 watt bulb for easier, more reliable detection than weaker models. Laboratory manual in general microbiology for undergraduate students.
Lamps that produce uv light in a more limited range may be better suited for more specific applications. Validation of the effectiveness of uv light in water system. This chapter describes common laboratory procedures that can reduce the risk of culture con. For courses in microbiology lab and nursing and allied health microbiology lab. There must be a log book to maintain the number of hours for which the uv. The goal of the laboratory is to expose students to the wide variety of lives in the microbial world. In microbiology lab, it is used for maintaining ph of the medium and diluents. Portable and benchtop ultraviolet lamps produce uv light in a range of wavelengths and are useful for detection, disinfection, and other laboratory applications for general functionality, consider using multipurpose lamps that produce light throughout the ultraviolet range. Its used for drinking and wastewater treatment, air disinfection, the. Ultraviolet germicidal irradiation uvgi is a disinfection method that uses shortwavelength ultraviolet ultraviolet c or uvc, light to kill or inactivate microorganisms by destroying nucleic acids and. Cordless and compact flashlights and handheld uv lamps for laboratory applications. In the lab experiment today, we want to find the effects of uv irradiation on two species of bacteria serratia marcescens, a gram negative rod and staphylococcus aureus, a gram positive coccus with. Uv lamp 1 lab bhi plates 4group halflids 1 lab sterile droppers 2group sterile swabs 2. Choose from 126 different sets of uv microbiology lab flashcards on quizlet.
A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Shapes, sizes, and features of uv room disinfection devices vary. Analyzing differences in bacterial optical density measurements between spectrophotometers brian c. Cell protection against uv damage include the repair mechanisms, such as the sos system, which enzymatically removes dimmers and inserts in their place new pyrimidine molecules. The most likely sources of uv light in the lab setting include. Control of microbial growth by radiation and antibiotics. As a trusted uv lamp supplier we have ce rated uv lamps for sale in the european union. Regarding proper clothing, legs and feet must be covered. Dr6000 laboratory spectrophotometer hach usa downloads. Position the handle end of the wire in the light blue cone of the flame. Many of the ultraviolet uv sources used in the laboratory emit high intensities of uv light, capable of producing painful eye and skin burns. Uv sources used in the laboratory emit high intensities of uv light, capable of producing painful eye and skin burns. Weve built our reputation on consistent, comprehensive mastery of daytoday operations, allowing.
Learn uv microbiology lab with free interactive flashcards. Monitoring ultraviolet lamps in biological safety cabinets with. Book september 2012 with,937 reads how we measure reads. Regarding lab attire, lab coats, gloves, and safety glasses will be provided. This lab exposes students to various methods heat, uv radiation, antibiotics and. Samples off and pt were collected before any exposure to uv light with the aim of evaluating the effect of the pump alone on bacterial contaminants. Loopmediated isothermal amplification lamp is a singletube technique for the amplification of dna and a lowcost alternative to detect certain diseases. Prolonged exposure to uv light helps ensure complete killoff of all microorganisms. The grade for general microbiology is based on a total of 800 points 600 from lecture, 200 from lab. This training presentation provides information about the. Uv has been used for disinfection since the mid20th century, with beginnings even earlier when sunlight was investigated for bactericidal effects in the mid19th century. Portable and benchtop ultraviolet lamps produce uv light in a range of wavelengths and are useful for detection, disinfection, and other laboratory applications for general functionality, consider using.